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August 15, 2017
DOI:
1Department of Metabolic Biology,John Innes Centre, Norwich Research Park, 2Department of Botany,University of Wisconsin, Madison, 3Department of Biochemistry and Molecular Biology,Saitama University, 4Precursory Research for Embryonic Science and Technology (PRESTO),Japan Science and Technology Agency (JST), 5Department of Cell and Developmental Biology,John Innes Centre, Norwich Research Park
Summary
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Este protocolo descreve um método simples para análise de sinais de cálcio em plantas geradas pela alimentação hemipteran insetos, como pulgões. Arabidopsis thaliana transformada com o biosensor de cálcio GFP GCaMP3 permitem a imagem em tempo real na vivo da dinâmica de cálcio com alta resolução temporal e espacial.
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Vincent, T. R., Canham, J., Toyota, M., Avramova, M., Mugford, S. T., Gilroy, S., Miller, A. J., Hogenhout, S., Sanders, D. Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor. J. Vis. Exp. (126), e56142, doi:10.3791/56142 (2017).
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