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Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
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JoVE Journal Medicine
Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

DOI:
10.3791/56320-v

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10:42 min

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December 31, 2017

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, ,
1Division of Oral Surgery and Orthodontics, Department of Dental Medicine and Oral Health,Medical University of Graz, 2Division of Preventive and Operative Dentistry, Periodontology, Prosthodontics and Restorative Dentistry, Department of Dental Medicine and Oral Health,Medical University of Graz

Chapters

  • 00:00Title
  • 01:26Instruments and material
  • 02:02Child preparation
  • 04:03Biofilm sampling
  • 06:00Transfer and storage
  • 06:40Application
  • 09:07Conclusion

Summary

Automatic Translation

Automatic Translation

Changes in the oral microbiome throughout childhood are of growing interest. Comparison of different microbiome studies reveals a lack of standardized sampling protocols. Limited space makes sampling the sound subgingival sulcus of children challenging. Paper point sampling is presented here in detail as the method of choice for this area.

Tags

Keywords: Oral BiofilmMicrobiome AnalysisHealthy ChildrenStandardized Sampling MethodSubgingival SulcusVestibular FoldSalivaSterile InstrumentsPaper Point SamplingPlaque DisclosureElectric ToothbrushCotton SwabsJunctional Epithelium

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