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December 26, 2017
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Summary
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Current bilayer culture models do not allow for functional in vitro studies that mimic in vivo microenvironments. Using polyethylene glycol and a zinc oxide templating method, this protocol describes the development of an ultrathin biomimetic basement membrane with tunable stiffness, porosity, and biochemical composition that closely mimics in vivo extracellular matrices.
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Cite this Article
Pellowe, A. S., Lauridsen, H. M., Matta, R., Gonzalez, A. L. Ultrathin Porated Elastic Hydrogels As a Biomimetic Basement Membrane for Dual Cell Culture. J. Vis. Exp. (130), e56384, doi:10.3791/56384 (2017).
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