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JoVE Journal
Bioengineering

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Ultrathin Porated Elastic Hydrogels As a Biomimetic Basement Membrane for Dual Cell Culture
 

Ultrathin Porated Elastic Hydrogels As a Biomimetic Basement Membrane for Dual Cell Culture

Article DOI: 10.3791/56384
December 26th, 2017

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Summary December 26th, 2017

Current bilayer culture models do not allow for functional in vitro studies that mimic in vivo microenvironments. Using polyethylene glycol and a zinc oxide templating method, this protocol describes the development of an ultrathin biomimetic basement membrane with tunable stiffness, porosity, and biochemical composition that closely mimics in vivo extracellular matrices.

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