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Indtagelse af fluorescerende, magnetiske nanopartikler til bestemmelse af væske-uptake evner i insekter
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JoVE Journal Biology
The Ingestion of Fluorescent, Magnetic Nanoparticles for Determining Fluid-uptake Abilities in Insects

Indtagelse af fluorescerende, magnetiske nanopartikler til bestemmelse af væske-uptake evner i insekter

DOI:
10.3791/56619-v

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07:03 min

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December 20, 2017

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, , , , , , ,
1Department of Biological Sciences,Kent State University at Stark, 2Department of Mathematical Sciences,Clemson University, 3Liquid Crystal Institute,Kent State University, 4Brookhaven National Laboratory

Chapters

  • 00:05Title
  • 00:38Insect Species, Preparation of Solutions, and Feeding Station Setup
  • 01:43Feeding Protocol
  • 03:05Dissections
  • 04:31Testing for Ingested Nanoparticles
  • 05:24Results: A Relationship Between Feeding Pore Size and Feeding Conduit Size
  • 06:28Conclusion

Summary

Automatic Translation

Automatic Translation

Væske-fodring insekter har mulighed for at erhverve minut mængder af væsker fra porøse overflader. Denne protokol beskriver en metode til at bestemme direkte mulighed for insekter at indtage væske fra porøse overflader ved hjælp af fodring løsninger med fluorescerende, magnetiske nanopartikler.

Tags

Fluorescent Magnetic NanoparticlesFluid-feeding InsectsInsect Mouth PartsInsect Feeding MechanismsBlue Bottle FliesHouse FliesCabbage ButterfliesSucrose SolutionFeeding StationNylon Net FiltersMembrane FiltersMicroscope SlidesManipulatorFeeding StageFilter PaperInsect Pin

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