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Etablering af en ekstracellulære sure pH kultur System
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JoVE Journal Biology
Establishment of an Extracellular Acidic pH Culture System

Etablering af en ekstracellulære sure pH kultur System

DOI:
10.3791/56660-v

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09:41 min

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November 19, 2017

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1Innovative Technology Laboratories,Kyowa Hakko Kirin Co., Ltd, 2Laboratory for Systems Biology and Medicine, RCAST,The University of Tokyo

Chapters

  • 00:05Title
  • 00:57Preparation of Acidic Culture Medium
  • 02:50Harvesting and Treating Cells
  • 04:31RNA Isolation
  • 06:30cDNA Synthesis and Analysis
  • 08:01Results: Transcriptional Upregulation of Acidic responsive Genes in Response to Lactic Acidosis and HCl-mediated Acidosis
  • 08:49Conclusion

Summary

Automatic Translation

Automatic Translation

Sure tumor mikromiljø spiller vigtige roller i tumor progression. For at vurdere virkningerne af sure ekstracellulære pH på kræft celler in vitro etableret vi enkle sure kultur systemer.

Tags

Extracellular Acidic PHTumor MicroenvironmentCancer ProgressionMalignancyAcidosisDMEMSodium BicarbonateL-glutaminePenicillin-streptomycinFetal Bovine SerumLactateHClPH MeasurementCell CulturePBSTrypsin

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