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Estabelecimento de um pH extracelular ácido sistema de cultura
JoVE Journal
Biology
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JoVE Journal Biology
Establishment of an Extracellular Acidic pH Culture System
DOI:

09:41 min

November 19, 2017

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Chapters

  • 00:05Title
  • 00:57Preparation of Acidic Culture Medium
  • 02:50Harvesting and Treating Cells
  • 04:31RNA Isolation
  • 06:30cDNA Synthesis and Analysis
  • 08:01Results: Transcriptional Upregulation of Acidic responsive Genes in Response to Lactic Acidosis and HCl-mediated Acidosis
  • 08:49Conclusion

Summary

Automatic Translation

O microambiente do tumor ácido tem um papel crítico na progressão do tumor. Para avaliar os efeitos do pH extracelular ácido sobre câncer células in vitro, estabelecemos sistemas simples cultura ácidas.

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