Journal
/
/
Mise en place d’un pH extracellulaire acide système de Culture
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Establishment of an Extracellular Acidic pH Culture System
DOI:

09:41 min

November 19, 2017

,

Chapters

  • 00:05Title
  • 00:57Preparation of Acidic Culture Medium
  • 02:50Harvesting and Treating Cells
  • 04:31RNA Isolation
  • 06:30cDNA Synthesis and Analysis
  • 08:01Results: Transcriptional Upregulation of Acidic responsive Genes in Response to Lactic Acidosis and HCl-mediated Acidosis
  • 08:49Conclusion

Summary

Automatic Translation

Le microenvironnement tumoral acide joue un rôle crucial dans la progression tumorale. Afin d’évaluer les effets des acides pH extracellulaire sur le cancer des cellules in vitro, nous avons établi des systèmes de culture acides simples.

Related Videos

Read Article