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Journal / Immunology and Infection / En lettvinte protokoll for å generere…
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
En lettvinte protokoll for å generere Site-Specifically Acetylated proteiner i Escherichia Coli
Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood
Journal (Immunology and Infection)
Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood
Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method
Journal (Immunology and Infection)
Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method
 
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En lettvinte protokoll for å generere Site-Specifically Acetylated proteiner i Escherichia Coli

Article DOI: 10.3791/57061-v • 11:09 min • December 9th, 2017
December 9th, 2017 •
1,2, 3, 4, 1, 1,2
1Department of Chemistry and Biochemistry, University of Arkansas, 2Cell and Molecular Biology Program, University of Arkansas, 3Department of Biological Sciences, University of Arkansas, 4Fayetteville High School

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Genetisk kode utvidelse fungerer som et kraftig verktøy for å studere en rekke biologiske prosesser, herunder protein acetylation. Her viser vi en lettvinte protokoll for å utnytte denne teknikken for å generere homogent acetylated proteiner ved bestemte områder i Escherichia coli celler.

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Immunologi problemet 130 Protein acetylation post-translasjonell modifikasjon genetiske koden ekspansjon noncanonical aminosyrer malate dehydrogenase syntetisk biologi
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