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Journal / Immunology and Infection / Overekspresjon og rensing av menneske
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Immunology and Infection

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Overekspresjon og rensing av menneske

Article DOI: 10.3791/56430-v • 07:35 min • August 3rd, 2017
August 3rd, 2017 •
*1, *1, 2, 2, 1,3, 1,3, 2, 2,4, 2
1Sackler Faculty of Medicine, Tel Aviv University, 2Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, 3Department of Ophthalmology, Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine, Tel Aviv University, 4Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine, Tel Aviv University
* These authors contributed equally

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En enkel protokoll for overekspresjon og rensing av kodonoptimalisert human cis- fenyltransferase under ikke-denaturerende betingelser fra Escherichia Coli , er beskrevet, sammen med en enzymatisk aktivitetsanalyse. Denne protokollen kan generaliseres for produksjon av andre cis- prenyltransferase proteiner i kvantitet og kvalitet egnet for mekanistiske studier.

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Immunologi utgave 126 kodonoptimalisering heterolog overekspresjon proteinrensing prenyltransferase dehydrodolichyldifosfat-syntase polyprenyl
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