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Medicine
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慢性的な痛みの治療に遺伝子組換えタンパク質の開発
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Medicine
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JoVE Journal
Medicine
Development of Recombinant Proteins to Treat Chronic Pain
Please note that all translations are automatically generated.
Click here for the English version.
慢性的な痛みの治療に遺伝子組換えタンパク質の開発
DOI:
10.3791/57071-v
•
10:37 min
•
April 11, 2018
•
Judith Prado
,
Jelena Popov-Celeketic
,
Cristine Steen-Louws
,
Ramin Raoof
,
Eric Hack
,
Niels Eijkelkamp
3
1
Laboratory of Translational Immunology
,
University Medical Center Utrecht, Utrecht University
,
2
Department of Rheumatology & Clinical Immunology
,
University Medical Center Utrecht, Utrecht University
,
3
Laboratory of Neuroimmunology and Developmental Origins of Disease
,
University Medical Center Utrecht, Utrecht University
Chapters
00:05
Title
01:15
Cell Transfection, Protein Purification, and Affinity Chromatrography
03:49
Bioactivity of IL4-10 Fusion Protein
04:46
Induction of Chronic Inflammatory Pain in Mice. Pain Measurements: The Von Frey Test: Mechanical Sensitivity
05:52
Pain measurements: Hargreaves Test for Thermal Sensitivity Dynamic Weight Bearing Test for Postural Deficits
06:48
Intrathecal Injection of IL4-10 Fusion Protein for Analgesia
07:56
Results: Persistent Inflammatory Pain Reduction Using IL4-10 Fusion Protein
09:38
Conclusion
Summary
Automatic Translation
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Automatic Translation
本稿で生産の方法と IL4 10 融合組換えタンパク質の品質管理のための詳細を提供します。また炎症性疼痛モデルマウスの痛みを解決するには、このタンパク質の有効性をテストする方法を示します。
Tags
Recombinant Proteins
IL4-10 Fusion Protein
Chronic Pain
Anti-inflammatory Cytokines
Protein Production
Protein Purification
HEK 293F Cells
Transfection
Affinity Chromatography
Dialysis
SDS-PAGE
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