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Cancer Research

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JoVE Journal Cancer Research
18F-FDG PET/CT Imaging og kvantitative Histology måle dynamiske endringer i glukose metabolisme i musen modeller av lungekreft
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18F-FDG PET/CT Imaging og kvantitative Histology måle dynamiske endringer i glukose metabolisme i musen modeller av lungekreft

Article DOI: 10.3791/57167-v • 06:51 min • July 21st, 2018
July 21st, 2018 •
1, 2, 3, 3, 3, 1, 4, 5, 1
1Division of Pulmonary and Critical Care Medicine, University of California Los Angeles David Geffen School of Medicine, 2University of North Carolina at Chapel Hill, 3Department of Molecular and Medical Pharmacology, University of California Los Angeles, 4Andor Technology, 5Division of Orthopaedic Surgery, University of California Los Angeles David Geffen School of Medicine

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I denne protokollen beskriver vi hvordan å utnytte [18F]-2-fluoro-2-deoxy-D-glucose fantes et positron utslipp tomografi og beregnede tomografi (18F-FDG PET/CT) imaging for å måle svulst metabolsk respons på målrettet terapi MLN0128 ved en Kras/Lkb1 mutant mus modell av lungekreft og kombinert bildebehandling med høy oppløsning ex vivo autoradiography og kvantitative histology.

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Kreftforskning problemet 137 18F-FDG PET bildebehandling lungekreft Glykolysen LKB1 KRAS mTOR
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