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用高频超声 (30/45 mhz) 系统对胎儿小鼠进行心血管成像

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Developmental Biology

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JoVE Journal Developmental Biology

Fetal Mouse Cardiovascular Imaging Using a High-frequency Ultrasound (30/45MHZ) System

Please note that all translations are automatically generated. Click here for the English version.

用高频超声 (30/45 mhz) 系统对胎儿小鼠进行心血管成像

DOI:

10.3791/57210-v

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07:34 min

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May 05, 2018

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Marlin Touma²

¹Neonatal/Congenital Heart Laboratory, Cardiovascular Research Laboratory, David Geffen School of Medicine,University of California, Los Angeles, ²Children’s Discovery and Innovation Institute, Department of Pediatrics, David Geffen School of Medicine,University of California, Los Angeles

Chapters

00:05Title
00:45Preparing the High Frequency Ultrasound Imaging System
01:36Preparing the Pregnant Mouse
02:35Embryo Identification
03:09Fetal Heart Visualization and Annotation
05:05Evaluating Cardiopulmonary Flow Parameters
05:44Evaluating the Feto-placental Axis
06:30Results: Hemodynamic Parameters of Normoxic and Hypoxic Fetal Mice at Gestational Day 18.5
06:56Conclusion

Summary

Automatic Translation

高频超声成像的胎儿小鼠有改进的影像分辨率, 可以提供精确的非侵入性的心脏发育和结构缺陷的特点。此处概述的协议旨在执行实时的胎儿小鼠超声心动图在体内。

Tags

Keywords: Fetal Mouse Cardiovascular Imaging High-frequency Ultrasound Cardiac Morphology Flow Indices Congenital Heart Defects 30/45MHz 2D Dynamic Imaging Anesthesia Electrocardiography Temperature Monitoring Fur Removal Fetal Orientation

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