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Isolation of Cerebral Capillaries from Fresh Human Brain Tissue
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JoVE Journal Neuroscience
Isolation of Cerebral Capillaries from Fresh Human Brain Tissue

Isolation of Cerebral Capillaries from Fresh Human Brain Tissue

DOI:
10.3791/57346-v

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06:35 min

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September 12, 2018

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, , , , , , , ,
1Sanders-Brown Center on Aging, Department of Pharmacology and Nutritional Sciences,University of Kentucky, 2Department of Pharmaceutical Sciences, College of Pharmacy,University of Kentucky, 3Department of Neuroscience,University of Kentucky

Chapters

  • 00:04Title
  • 00:34Brain Sample Preparation
  • 02:41Filtration and Capillary Collection
  • 05:14Results: Isolated Human Brain Capillary
  • 05:40Conclusion

Summary

Automatic Translation

Automatic Translation

Isolated brain capillaries from human brain tissue can be used as a preclinical model to study barrier function under physiological and pathophysiological conditions. Here, we present an optimized protocol to isolate brain capillaries from fresh human brain tissue.

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Keywords: Cerebral CapillariesBlood-brain BarrierIsolationHomogenizationFicoll Density GradientFiltrationCentrifugation

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