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Double Fluorescence in situ Hybridization in Fresh Brain Sections
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Neuroscience
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JoVE Journal Neuroscience
Double Fluorescence in situ Hybridization in Fresh Brain Sections

Double Fluorescence in situ Hybridization in Fresh Brain Sections

DOI:
10.3791/2102-v

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12:15 min

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August 14, 2010

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, , ,
1Department of Brain and Cognitive Sciences,University of Rochester, 2Center for Visual Science,University of Rochester

Chapters

  • 00:04Title
  • 00:07Introduction
  • 00:56Prep of Sephadex G-50 Columns
  • 02:54Labeling and Purification of Riboprobes
  • 04:25Post-fixation, Acetylation, and Hybridization
  • 07:22Detection and Visualization of Riboprobes
  • 10:35Representative Results
  • 11:35Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol involves a non-radioactive in-situ hybridization procedure that enables the simultaneous identification of two transcript species, at a single cell resolution, in thin sections of the vertebrate brain.

Tags

Double Fluorescence In Situ HybridizationDFISHMRNAFresh Frozen Brain SectionsGene Co-regulationAnatomical OrganizationNeurochemical PropertiesSensory ExperienceCentral Sensory CircuitsSingle Cell ResolutionProtocolValidationMiceRatsSongbirdsVertebrate Species

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