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JoVE Journal
Neuroscience

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Double Fluorescence in situ Hybridization in Fresh Brain Sections
 

Double Fluorescence in situ Hybridization in Fresh Brain Sections

Article DOI: 10.3791/2102
August 14th, 2010

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Summary August 14th, 2010

This protocol involves a non-radioactive in-situ hybridization procedure that enables the simultaneous identification of two transcript species, at a single cell resolution, in thin sections of the vertebrate brain.

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