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Methods for Study of Neuronal Morphogenesis: Ex vivo RNAi Electroporation in Embryonic Murine Cerebral Cortex
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Chapters
- 00:05Title
- 01:53Dissection and Electroporation
- 04:10Embedding and Sectioning Electroporated Cortices
- 06:53Culturing and Analysis of Organotypic Slices
- 08:55Images of Electroporated Neurons
- 09:35Conclusion
To conduct a rapid assessment of the function of genes in the development of cerebral cortex, we describe methods involving the ex vivo electroporation of plasmids co-expressing inhibitory RNA (RNAi) and GFP in murine embryonic cortex. This protocol is amenable to the study of various aspects of neurodevelopment such as neurogenesis, neuronal migration and neuronal morphogenesis including dendrite and axon outgrowth.
Tags
Neuronal MorphogenesisEx Vivo RNAi ElectroporationEmbryonic Murine Cerebral CortexCerebral CortexHigher Cognitive FunctionsSix Layered StructureInside-first Outside-last MannerNeuronal MigrationRegulation Of Neuronal MorphogenesisPrimary CulturesOrganotypic SlicesPSilencer Modified VectorU6 PromoterDouble Stranded Hairpin RNACMV PromoterGFP ProteinNeurite OutgrowthCandidate GenesMosaic Analysis
