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Isolering af entero Glial celler fra Submucosa og Lamina Propria af voksen mus
 

Isolering af entero Glial celler fra Submucosa og Lamina Propria af voksen mus

Article doi: 10.3791/57629
August 15th, 2018 Usage Statistics

Summary August 15th, 2018

Please note that all translations are automatically generated.

Click here for the English version.

Her beskriver vi isolering af entero-glial celler fra den tarm-submucosa bruge sekventielle EDTA inkubationer til Chelat divalent kationer og derefter inkubation i ikke-enzymatiske celle opsving løsning. Plating den resulterende cellesuspension på poly-D-Lysin og laminin resulterer i en yderst højberiget kultur af submukøse glial celler for funktionelle analyse.

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