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Utilizing the Precision-Cut Lung Slice to Study the Contractile Regulation of Airway and Intrapulmonary Arterial Smooth Muscle
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JoVE Journal Biology
Utilizing the Precision-Cut Lung Slice to Study the Contractile Regulation of Airway and Intrapulmonary Arterial Smooth Muscle

Utilizing the Precision-Cut Lung Slice to Study the Contractile Regulation of Airway and Intrapulmonary Arterial Smooth Muscle

DOI:
10.3791/63932-v

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08:59 min

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May 05, 2022

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,
1Division of Newborn Medicine, Department of Pediatrics,Massachusetts General Hospital and Harvard Medical School

Chapters

  • 00:04Introduction
  • 00:47Inflation of Mouse Lungs
  • 03:25Sectioning of Lung Lobes
  • 04:09Analyzing Contractile Responses of Intrapulmonary Airways and Arteries
  • 05:08Analyzing Ca2+ Signaling of Airway or Vascular Smooth Muscle Cells
  • 06:45Results: Analyses of Airways and Pulmonary Arteries in Mouse Precision-Cut Lung Slices
  • 08:07Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes preparing and utilizing mouse precision-cut lung slices to assess the airway and intrapulmonary arterial smooth muscle contractility in a nearly in vivo milieu.

Tags

Keywords: Precision-cut Lung SliceAirwayIntrapulmonary ArterialSmooth MuscleEx VivoPulmonary ResearchAgaroseGelatinTracheaPulmonary Vasculature

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