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Journal / Biochemistry / Påvisning af små GTPase Prenylation og GTP…
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Biochemistry
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JoVE Journal Biochemistry
Påvisning af små GTPase Prenylation og GTP bindende ved hjælp af membran fraktionering og GTPase-forbundet Immunosorbent Assay
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Påvisning af små GTPase Prenylation og GTP bindende ved hjælp af membran fraktionering og GTPase-forbundet Immunosorbent Assay

Article DOI: 10.3791/57646-v • 13:52 min • November 11th, 2018
November 11th, 2018 •
1,2, 1,2,3, 1, 1,2, 4, 5, 6,7,8, 1,9, 1,2,10
1Department of Human Anatomy and Cell Science, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, 2Biology of Breathing Theme, Children Hospital Research Institute of Manitoba, University of Manitoba, 3Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, 4Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, Center for Comparative Respiratory Biology and Medicine, 5Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, 6Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, 7Centre de biophysique moléculaire - UPR 4301, Centre national de la recherche scientifique (CNRS) CS80054, 8LinkoCare Life Sciences AB, 9College of Nursing and Children's Hospital Research Institute of Manitoba, Rady Faculty of Health Sciences, University of Manitoba, 10Health Policy Research Center, Institute of Health, Shiraz University of Medical Sciences

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Her beskriver vi en protokol for at undersøge prenylation og Guanosinmonofosfat-5'-trifosfat (GTP)-lastning af Rho GTPase. Denne protokol består af to detaljerede metoder, nemlig membran fraktionering og en GTPase-forbundet immunosorbent assay. Protokollen kan bruges til at måle prenylation og GTP indlæsning af forskellige andre små GTPases.

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Biokemi sag 141 Simvastatin glioblastom Rho GTPase cancer cellebiologi prenylation mevalonsyre vej
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