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JoVE Journal Biochemistry
Ikke-radioaktiv analyse til måling af polynukleotid phosphorylation af små nukleotid substrater
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Ikke-radioaktiv analyse til måling af polynukleotid phosphorylation af små nukleotid substrater

Article DOI: 10.3791/61258-v • 06:49 min • May 8th, 2020
May 8th, 2020 •
1, 1
1Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Department of Health and Human Services, National Institutes of Health

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Denne protokol beskriver en ikke-radioaktiv analyse til måling af kinase aktivitet af polynukleotidkinaser (PNKs) på små DNA- og RNA-substrater.

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Biokemi polynukleotid kinase polyarylamid gel phosphoryl overførsel reaktion RNA phosphorylation Grc3 ikke-radioaktiv analyse
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