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JoVE Journal Genetics
Bronchoalveolar Lavage Exosomes i LPS-induceret septisk lunge skade
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Bronchoalveolar Lavage Exosomes i LPS-induceret septisk lunge skade

Article DOI: 10.3791/57737-v • 08:27 min • May 21st, 2018
May 21st, 2018 •
1,2, 1, 1,2
1Department of Veterans Affairs, Atlanta VAMC, 2Division of Pulmonary and Critical Care Medicine, Department of Medicine, Emory University

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Mus udsættes for intraperitoneal LP'ER udskiller exosomes i bronchopneumoni-alveolære lavage (BAL) væske, som er pakket med miRNAs. Ved hjælp af en fælles kultur system, viser vi, at exosomes udgivet i BAL væske forstyrre udtryk for tight junction proteiner i bronchiale epitel celler og øge udtryk af pro-inflammatoriske cytokiner, at fremhæver lunge skade.

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Genetik spørgsmålet 135 Exosomes MicroRNA miR-155 miR-146 LP'ER lunge sepsis
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