Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

FM Dye Cycling at the Synapse

FM Dye Cycling at the Synapse: Comparing High Potassium Depolarization, Electrical and Channelrhodopsin Stimulation

Article DOI: 10.3791/57765 08:31 min
May 24th, 2018


Summary May 24th, 2018

Synaptic vesicle (SV) cycling is the core mechanism of intercellular communication at neuronal synapses. FM dye uptake and release are the primary means of quantitatively assaying SV endo- and exocytosis. Here, we compare all the stimulation methods to drive FM1-43 cycling at the Drosophila neuromuscular junction (NMJ) model synapse.


Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter