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Journal / Cancer Research / Analisi di sequenza di Gene dell'immunoglobulina…
JoVE Journal
Cancer Research

This content is Open Access.

JoVE Journal Cancer Research
Analisi di sequenza di Gene dell'immunoglobulina nella leucemia linfocitaria cronica
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Analisi di sequenza di Gene dell'immunoglobulina nella leucemia linfocitaria cronica: Da materiale paziente all'interpretazione di sequenza

Article DOI: 10.3791/57787-v • 09:02 min • November 26th, 2018
November 26th, 2018 •
*1, *2,3, 1, 4, 5, 6, 7, 2,3, 1,2, 8
1Institute of Applied Biosciences, Centre for Research and Technology Hellas, 2Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, 3Department of Molecular Medicine and Surgery, Karolinska Institutet, 4Division of Immunology, Transplantation and Infectious, IRCCS San Raffaele Scientific Institute, 5Department of Immunology, Laboratory for Medical Immunology, Erasmus University Medical Center, 6Hematology Department, Nikea General Hospital, 7Assistance publique - Hôpitaux de Paris (AP-HP), Hopital Pitié-Salpêtrière, Department of Hematology, and UPMC University Paris 06, UMRS 1138, 8Division of Experimental Oncology, IRCCS Istituto Scientifico San Raffaele and Università Vita-Salute San Raffaele
* These authors contributed equally

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Summary
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Qui, presentiamo un protocollo che dettagli gli aspetti tecnici e requisiti essenziali affinché robusta analisi di sequenza del gene di IG in pazienti con la leucemia linfocitaria cronica (CLL), basato sull'esperienza accumulata dell'iniziativa di ricerca europea su CLL (ERIC).

Tags

Ricerca sul cancro problema 141 leucemia linfocitaria cronica (CLL) ipermutazione somatica (SHM) immunoglobuline variabile pesante dell'immunoglobulina (IGHV) complementarità determinazione regione 3 (CDR3) sistema di informazione internazionale ImMunoGeneTics (IMGT)
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