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Sund hjerne-hypofyse skiver for elektrofysiologiske undersøgelser af hypofyse celler i Teleost fisk
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish
DOI:

07:14 min

August 16, 2018

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Chapters

  • 00:04Title
  • 00:47Tissue Harvest and Section Acquisition
  • 03:06Perforated Patch-clamp and Electrophysiological Recordings
  • 05:39Results: Representative Pituitary Cell Current Clamp Recordings
  • 06:49Conclusion

Summary

Automatic Translation

Artiklen beskriver en optimeret protokol for at gøre levedygtige hjerne-hypofyse væv skiver, ved hjælp af teleost fisk medaka (Oryzias latipes), efterfulgt af elektrofysiologiske optagelser af hypofyse celler ved hjælp af patch-clamp-teknik med den perforeret patch configuration.

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