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Journal
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Biochemistry
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操纵活细胞构建稳定的3D 蜂窝组件, 无需人工支架
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold
Please note that all translations are automatically generated.
Click here for the English version.
操纵活细胞构建稳定的3D 蜂窝组件, 无需人工支架
DOI:
10.3791/57815-v
•
07:09 min
•
October 26, 2018
•
Takehiro Yamazaki*
1
,
Hiroaki Taniguchi*
2
,
Shoto Tsuji
,
Shiho Sato
,
Takahiro Kenmotsu
,
Kenichi Yoshikawa
,
Koichiro Sadakane
1
Faculty of Life and Medical Sciences
,
Doshisha University
,
2
The Institute of Genetics and Animal Breeding
,
Polish Academy of Sciences
Chapters
00:04
Title
00:48
Preparation of Cells
02:41
Preparation of Dextran
03:18
Preparation for Laser and Microscopy
03:55
Cell Manipulation Using the Laser Trapping System
05:30
Construction of a 3-Dimensional Cell Structure
06:10
Results: Analysis Stable 3D Cellular Assembly
06:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们展示了一种新的方法来构建一个基于单细胞的3维 (3D) 装配体, 而无需人工脚手架。
Tags
3D Cellular Assembly
Cell Manipulation
Hydrophilic Polymer
Dextran
NAMRU Mouse Mammary Gland Epithelial Cells
Trypsin
DMEM
FBS
Penicillin-Streptomycin
Centrifugation
Cryopreservation
Laser
Camera
LED
Focus Adjust
Moving Stage
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