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哺乳动物 Bestrophin 离子通道的表达与纯化
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Biochemistry
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JoVE Journal Biochemistry
Expression and Purification of Mammalian Bestrophin Ion Channels
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哺乳动物 Bestrophin 离子通道的表达与纯化

DOI:
10.3791/57832-v

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08:12 min

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August 02, 2018

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, , , ,
1Department of Pharmacology and Physiology,University of Rochester, School of Medicine and Dentistry

Chapters

  • 00:04Title
  • 00:43Protein Expression
  • 03:00Protein Purification
  • 07:02Results: Expression and Purification of a Mammalian Bestrophin Protein
  • 07:32Conclusion

Summary

Automatic Translation

Automatic Translation

离子通道的纯化往往具有挑战性, 但一旦达到, 它就有可能允许对通道的功能和结构进行体外调查。在这里, 我们描述的步骤, 以表达和纯化哺乳动物 bestrophin 蛋白, 一个家庭的 Ca2 +激活 Cl 通道.

Tags

Mammalian Bestrophin Ion ChannelsProtein ExpressionPurificationHEK293F CellsBaculovirusSodium ButyrateCell DensityCell ViabilityProtein Purification

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