Long-term <em>In Vivo</em> Tracking of Inflammatory Cell Dynamics Within <em>Drosophila</em> Pupae

Helen Weavers; Anna Franz; Will Wood; Paul Martin

doi:10.3791/57871

Journal

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Immunology and Infection

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Op lange termijn In Vivo Tracking van inflammatoire cel dynamiek binnen Drosophila poppen

Long-term <em>In Vivo</em> Tracking of Inflammatory Cell Dynamics Within <em>Drosophila</em> Pupae

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Long-term In Vivo Tracking of Inflammatory Cell Dynamics Within Drosophila Pupae

Op lange termijn In Vivo Tracking van inflammatoire cel dynamiek binnen Drosophila poppen

Please note that all translations are automatically generated. Click here for the English version.

8,697 Views

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09:26 min

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June 14, 2018

DOI:

10.3791/57871-v

DOI:

10.3791/57871-v

•

09:26 min

June 14, 2018

•

4 Views

Helen Weavers^1,2, Anna Franz¹, Will Wood³, Paul Martin^1,4

¹School of Biochemistry, Biomedical Sciences,University of Bristol, ²School of Cellular and Molecular Medicine, Biomedical Sciences,University of Bristol, ³MRC Centre for Inflammation Research,University of Edinburgh, Queens Medical Research Institute, ⁴School of Physiology, Pharmacology, and Neuroscience, Biomedical Sciences,University of Bristol

Transcript

Summary

Hier presenteren we een protocol voor live-imaging wond reparatie en de geassocieerde inflammatoire respons bij hoge spatio-temporele resolutie in vivo. Deze methode maakt gebruik van de popstadium van Drosophila ontwikkeling om op lange termijn beeldvormings- en na verloop van tijd het volgen van specifieke cel populaties en is compatibel met efficiënte RNAi-gemedieerde gene inactivatie.