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Journal / Genetics / 原位杂交法在小鼠心脏切片组织特异 miRNA…
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Genetics

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JoVE Journal Genetics
原位杂交法在小鼠心脏切片组织特异 miRNA 表达谱分析中的应用
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原位杂交法在小鼠心脏切片组织特异 miRNA 表达谱分析中的应用

Article DOI: 10.3791/57920-v • 08:22 min • September 15th, 2018
September 15th, 2018 •
1, 2, 3
1Department of Cell and Developmental Biology, University College London, 2Yale Cardiovascular Research Group, Section of Cardiovascular Medicine, Yale School of Medicine, 3Yale Cardiovascular Research Center, Section of Cardiovascular Medicine, Yale School of Medicine

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微 rna (miRNAs) 是短而高度同源的 RNA 序列, 充当信使 rna (基因) 的转录后调节剂。目前 miRNA 检测方法的灵敏度和特异性各不相同。我们描述了一个协议, 结合原位杂交和染色的同时检测 miRNA 和蛋白质分子在小鼠心脏组织切片。

Tags

遗传学 问题 139 微 RNA 表达谱 低噪声放大探针,原位杂交 染色 心肌肥厚
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