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JoVE Journal
Genetics

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原位杂交法在小鼠心脏切片组织特异 miRNA 表达谱分析中的应用
 

原位杂交法在小鼠心脏切片组织特异 miRNA 表达谱分析中的应用

Article doi: 10.3791/57920
September 15th, 2018 Usage Statistics

Summary September 15th, 2018

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微 rna (miRNAs) 是短而高度同源的 RNA 序列, 充当信使 rna (基因) 的转录后调节剂。目前 miRNA 检测方法的灵敏度和特异性各不相同。我们描述了一个协议, 结合原位杂交和染色的同时检测 miRNA 和蛋白质分子在小鼠心脏组织切片。

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