Journal
/
/
酵母神经退行性蛋白病模型中组蛋白后转化修饰改变的表征
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models
DOI:

08:33 min

March 24, 2019

, , , , , ,

Chapters

  • 00:04Title
  • 01:00Neurodegenerative Proteinopathy-associated Protein Overexpression in S. cerevisiae
  • 03:02Cell Lysis and Protein Transfer
  • 05:28Histone Post-translational Modification Detection
  • 06:52Results: Representative Effects of Yeast Protein Overexpression on Histone Expression and Modification
  • 08:03Conclusion

Summary

Automatic Translation

该协议概述了实验程序, 以描述全基因组的变化, 在与 ALS 和帕金森病相关的蛋白质过度表达的情况下, 组蛋白翻译后修饰 (PTM) 的水平发生。酿酒酵母模型。SDS-PAGE 分离后, 通过西方印迹, 通过修正特异性抗体检测单个组蛋白 PTM 水平。

Related Videos

Read Article