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Journal
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Genetics
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利用南方印迹和聚合酶链反应鉴定小鼠胚胎干细胞的同源重组事件
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Genetics
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JoVE Journal
Genetics
Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
Please note that all translations are automatically generated.
Click here for the English version.
利用南方印迹和聚合酶链反应鉴定小鼠胚胎干细胞的同源重组事件
DOI:
10.3791/58467-v
•
08:01 min
•
November 20, 2018
•
Dan Zhou*
1,2
,
Lei Tan*
1
,
Jian Li*
3
,
Tanbin Liu
,
Yi Hu
,
Yalan Li
,
Sachiyo Kawamoto
,
Chengyu Liu
,
Shiyin Guo
,
Aibing Wang
1
Lab of Animal Models and Functional Genomics (LAMFG), The Key Laboratory of Animal Vaccine & Protein Engineering, College of Veterinary Medicine
,
Hunan Agricultural University (HUNAU)
,
2
Department of Pathology
,
Georgetown University Medical School
,
3
College of Food Science and Technology
,
Hunan Agricultural University (HUNAU)
,
4
Lab of Molecular Cardiology (LMC)
,
National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH)
,
5
Transgenic Core
,
National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH)
Chapters
00:04
Title
01:02
Southern Blotting Screening
04:40
Results: Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells
06:07
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个详细的协议, 以确定同源重组事件发生在小鼠胚胎干细胞使用南方印迹和/或 pcr。利用传统的胚胎干细胞同源重组介导靶向技术生成的非肌肉肌球蛋白 ii 基因替代小鼠模型就是例证。
Tags
Homologous Recombination
Mouse Embryonic Stem Cells
Southern Blotting
Polymerase Chain Reaction
Molecular Biology
Cellular Biology
Genetically Modified Cells
GDNA Digestion
Agarose Gel Electrophoresis
DNA Transfer
DNA Labeling
DNA Hybridization
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