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Journal
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Bioengineering
/
o 型血管哺乳动物细胞产生均匀集料的轨道晃动培养
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates
Please note that all translations are automatically generated.
Click here for the English version.
o 型血管哺乳动物细胞产生均匀集料的轨道晃动培养
DOI:
10.3791/57922-v
•
05:40 min
•
January 07, 2019
•
Ikki Horiguchi
2
,
Ikumi Suzuki
,
Takashi Morimura
,
Yasuyuki Sakai
4
1
Department of Chemical System Engineering
,
University of Tokyo
,
2
Department of Biotechnology
,
Osaka University
,
3
Biotech Business Unit
,
Fukoku Co. Ltd
,
4
Institute of Industrial Science
,
University of Tokyo
Chapters
00:04
Title
01:18
Seeding Cells in an O-shaped Bag
02:42
Medium Change
03:11
Cell Collection
04:07
Results: Morphology and Viability of Aggregates
05:18
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们提出了一个使用 o 型容器的协议, 专门用于悬浮培养的细胞集料, 轨道晃动。在这个袋子里生长的 hek293 细胞形成比传统培养容器中生长的细胞更均匀的聚集物。
Tags
Orbital Shaking Culture
Mammalian Cells
O-shaped Vessels
Uniform Aggregates
Drug Screening
Suspension Culture
HEK-293 Cells
Cell Aggregation
Cell Culture
Bioreactor Design
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