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En Orbital risting kultur pattedyrceller i O-formet fartøy å produsere Uniform aggregater
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates
DOI:

05:40 min

January 07, 2019

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Chapters

  • 00:04Title
  • 01:18Seeding Cells in an O-shaped Bag
  • 02:42Medium Change
  • 03:11Cell Collection
  • 04:07Results: Morphology and Viability of Aggregates
  • 05:18Conclusion

Summary

Automatic Translation

Her presenterer vi en protokoll for bruk O-formet fartøy, spesialisert for suspensjon kulturer av mobilnettet aggregat, med orbital risting. De HEK293 cellene vokst i denne vesken danner mer homogen aggregater enn de dyrket i tradisjonell kultur fartøy.

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