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小鼠骨髓 Oligopotent 和世系髓样祖细胞的鉴定与分离
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

小鼠骨髓 Oligopotent 和世系髓样祖细胞的鉴定与分离

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07:21 min

July 29, 2018

DOI:

07:21 min
July 29, 2018

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Summary

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我们展示了如何用磁性和荧光分选 (mac 和流动力学) 相结合的方法, 从小鼠骨髓中识别和分离出6个髓样祖细胞。本协议可用于体外培养测定 (纤维素或液体培养),体内移植实验, RNA/蛋白质分析。

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