JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
从小鼠中分离和培养骨髓衍生树突状细胞的经济有效的方案
JoVE Journal
Immunology and Infection
Author Produced
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice
Please note that all translations are automatically generated.
Click here for the English version.
从小鼠中分离和培养骨髓衍生树突状细胞的经济有效的方案
DOI:
10.3791/63125-v
•
04:29 min
•
July 01, 2022
•
Huiqin Tang*
1
,
Hui Xie*
1
,
Zhun Wang
,
Shuanghe Peng
,
Wenli Ni
,
Linpei Guo
1
Tianjin Institute of Urology
,
The Second Hospital of Tianjin Medical University
,
2
Department of Pathology
,
The Second Hospital of Tianjin Medical University
,
3
Department of Urology
,
The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University
Chapters
00:17
Introduction
00:37
Isolation of Bone Marrow and Preparation of BM Cells
02:05
Induction Culture of BMDC
03:11
Results
03:59
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里，我们提出了一种经济有效的方法，在用10 ng / mL GM-CSF / IL-4培养7天后从小鼠中分离和产生高纯度骨髓来源的树突状细胞。
Tags
Keywords: Bone Marrow-derived Dendritic Cells
Isolation
Culture
Mouse
BMDC
CD11c
CD80
MSC2
GM-CSF
IL-4
Cost-effective
Efficient
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/63125?article_type=v
弓形虫
囊肿壁形成激活骨髓衍生的巨噬细胞和Bradyzoite条件
check_url/63125?article_type=v
高效转染树突状细胞的无细胞的成熟优化的协议
check_url/63125?article_type=v
小鼠骨髓来源的树突状细胞与跟踪研究的Qdot纳米晶的生成及标签
check_url/63125?article_type=v
隔离和免疫淋巴细胞和树突状细胞的小鼠Peyer氏斑
check_url/63125?article_type=v
使用骨髓源性巨噬细胞巨噬细胞极化的调查
check_url/63125?article_type=v
从人类胸腺髓样树突状细胞和上皮细胞的分离
check_url/63125?article_type=v
骨髓衍生的巨噬细胞产
check_url/63125?article_type=v
隔离和小鼠骨髓的静脉注射单核细胞衍生
check_url/63125?article_type=v
从人类腭扁桃体B和T淋巴细胞有效隔离协议
check_url/63125?article_type=v
代和GM-CSF的鉴定源性肺泡状巨噬细胞和树突状细胞从小鼠骨髓
Read Article
