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用阴离子交换树脂检测 Bioaerosols 病毒
JoVE Journal
Environment
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JoVE Journal
Environment
Detection of Viruses from Bioaerosols Using Anion Exchange Resin
Please note that all translations are automatically generated.
Click here for the English version.
用阴离子交换树脂检测 Bioaerosols 病毒
DOI:
10.3791/58111-v
•
06:10 min
•
August 22, 2018
•
Joshua W. Schaeffer
,
Jeffrey C. Chandler
,
Margaret Davidson
3
,
Sheryl L. Magzamen
,
Alma Pérez-Méndez
,
Stephen J. Reynolds
,
Lawrence D. Goodridge
,
John Volckens
,
Alan B. Franklin
,
Susan A. Shriner
,
Bledar Bisha
1
High Plains Intermountain Center for Agricultural Health and Safety, Department of Environmental and Radiological Health Sciences
,
Colorado State University
,
2
National Wildlife Research Center, Wildlife Services, Animal and Plant Health Inspection Service
,
United States Department of Agriculture
,
3
Western Sydney University
,
4
Leprino Foods, Inc
,
5
Department of Food Science and Agricultural Chemistry
,
McGill University
,
6
Department of Mechanical Engineering
,
Colorado State University
,
7
Department of Animal Science
,
University of Wyoming
Chapters
00:04
Title
00:30
Bioaerosol Chamber Setup and Model Bioaerosolization Experiments
02:58
Nucleic Acid Extraction and qRT-PCR Detection
04:10
Results: Anion Exchange Resin Improves qRT-PCR-based, Negatively-charged Virus Bioaerosol Detection
04:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
证明了一种基于液体冲击的气溶胶病毒取样方法。当与下游分子检测相结合时, 该方法允许对 bioaerosols 的病毒进行简便、灵敏的检测。
Tags
Virus Detection
Bioaerosols
Anion Exchange Resin
Molecular Detection
Public Health
Veterinary Health
Viral Particles
Collision Nebulizer
Liquid Impingers
HEPA Filtered Air
Air Contaminants
Sampling
Calibration
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