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Journal / Biochemistry / Kovalente immobilisering av proteiner for ett…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Kovalente immobilisering av proteiner for ett molekyl Force spektroskopi
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Kovalente immobilisering av proteiner for ett molekyl Force spektroskopi

Article DOI: 10.3791/58167-v • 11:13 min • August 20th, 2018
August 20th, 2018 •
1,2,3, 2, 1,3, 3, 2, *4, *1,3
1Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, 2FG Protein Biochemistry & Cellular Microbiology, Munich University of Applied Sciences, 3Center for Nano Science, Ludwig-Maximilians-Universität München, 4Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie, University Medical Center Göttingen
* These authors contributed equally

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Denne protokollen beskriver kovalente immobilisering av proteiner med en heterobifunctional silane kopling agent silisium oksid overflater laget for atomic force mikroskopi basert ett molekyl force spektroskopi som er eksemplifisert ved den samspillet av RrgA (pilus-1 tips adhesin av S. pneumoniae) med fibronectin.

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Biokjemi problemet 138 Kovalente protein immobilisering overflate silanization heterobifunctional pinne reagens EDC/NHS kjemi primære Amin atomic force mikroskopi AFM ett molekyl force spektroskopi SMFS
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