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1GCC Center for Advanced Microscopy and Image Informatics, 2Department of Molecular and Cellular Biology,Baylor College of Medicine, 3Center for Translational Cancer Research, Institute of Biosciences and Technology,Texas A&M University, 4Department of Pharmacology and Chemical Biology,Baylor College of Medicine
Chapters
- 00:04Introduction
- 00:34RNA Fluorescence In Situ Hybridization (FISH) Fixation
- 01:14RNA FISH Hybridization
- 02:16Imaging Preparation
- 03:08High Resolution Microscopy and Image Processing
- 04:05Image Analysis
- 04:45Results: Representative Image Segmentation and E2-Induced GREB1 Quantification
- 05:56Conclusion
Enkeltmolekyl RNA fluorescens in situ hybridisering (smFISH) er en metode for å nøyaktig kvantifisere nivåer og lokalisering av spesifikke RNAer på enkeltcellenivå. Her rapporterer vi våre validerte laboratorieprotokoller for behandling, avbildning og bildeanalyse av våtbenker for encellet kvantifisering av spesifikke RNAer.
