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JoVE Journal Bioengineering
Ácido hialurónico basado en hidrogeles de 3 dimensiones cultura de células de Glioblastoma derivados del paciente
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Ácido hialurónico basado en hidrogeles de 3 dimensiones cultura de células de Glioblastoma derivados del paciente

Article DOI: 10.3791/58176-v • 08:35 min • August 24th, 2018
August 24th, 2018 •
1, 1, 1, 2
1Department of Bioengineering, University of California, Los Angeles, 2Department of Bioengineering, Jonsson Comprehensive Cancer Center, Broad Stem Cell Research Center, Brain Research Institute, University of California, Los Angeles

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Aquí, presentamos un protocolo para el cultivo tridimensional de células de glioblastoma derivados del paciente dentro de biomateriales ortogonalmente ajustables diseñados para imitar la matriz del cerebro. Este enfoque proporciona una en vitro, plataforma experimental que mantiene muchas características de en vivo células de glioblastoma normalmente perdidas en cultura.

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Bioingeniería número 138 ácido hialurónico cultura 3D glioblastoma microambiente matriz extracelular biomaterial mecanobiología resistencia a los medicamentos
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