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Bioengineering
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透明质酸基水凝胶对3维患者源性胶质瘤细胞培养的研究
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Bioengineering
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JoVE Journal
Bioengineering
Hyaluronic-Acid Based Hydrogels for 3-Dimensional Culture of Patient-Derived Glioblastoma Cells
Please note that all translations are automatically generated.
Click here for the English version.
透明质酸基水凝胶对3维患者源性胶质瘤细胞培养的研究
DOI:
10.3791/58176-v
•
08:35 min
•
August 24, 2018
•
Weikun Xiao
,
Arshia Ehsanipour
,
Alireza Sohrabi
,
Stephanie K. Seidlits
1
Department of Bioengineering
,
University of California, Los Angeles
,
2
Department of Bioengineering, Jonsson Comprehensive Cancer Center, Broad Stem Cell Research Center, Brain Research Institute
,
University of California, Los Angeles
Chapters
00:04
Title
00:31
Hydrogel Crosslinking and Cell Encapsulation
03:11
Hydrogel Culture Single Cell Extraction for Flow Cytometry and Cryopreservation for Sectioning
06:10
Results: Hyaluronic Acid-Based 3D Patient-Derived Glioblastoma Cell Hydrogel Cultures
07:55
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个协议, 三维培养病人的胶质瘤细胞在正交可调谐生物材料设计, 以模仿大脑基质。这种方法提供了一种
体外
实验平台, 它能维持
体内
胶质瘤细胞的许多特征, 通常在培养中丢失。
Tags
Hyaluronic Acid
Hydrogels
3D Cell Culture
Glioblastoma
Extracellular Matrix
Patient-derived Cells
Cell Encapsulation
PEG-Mal
Silicone Rubber Molds
Cell Dissociation
Cell Suspension
Gelation
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