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JoVE Journal Bioengineering
Hyaluronsyre baseret Hydrogels til 3-dimensionelle kultur af Patient-afledte glioblastom celler
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Hyaluronsyre baseret Hydrogels til 3-dimensionelle kultur af Patient-afledte glioblastom celler

Article DOI: 10.3791/58176-v • 08:35 min • August 24th, 2018
August 24th, 2018 •
1, 1, 1, 2
1Department of Bioengineering, University of California, Los Angeles, 2Department of Bioengineering, Jonsson Comprehensive Cancer Center, Broad Stem Cell Research Center, Brain Research Institute, University of California, Los Angeles

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Vi præsenterer her, en protokol for tre-dimensionelle kultur af patient-afledte glioblastom celler i retvinklet afstemmelige biomaterialer designet til at efterligne den hjerne matrix. Denne fremgangsmåde giver en in vitro-, eksperimentelle platform, der vedligeholder mange egenskaber i vivo glioblastom celler typisk tabt i kulturen.

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Bioteknologi spørgsmålet 138 hyaluronsyre 3D kultur glioblastom mikromiljø ekstracellulære matrix biomateriale mechanobiology resistens
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