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Detectie en isolatie van Apoptotic instanties hoge zuiverheid
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Detection and Isolation of Apoptotic Bodies to High Purity
DOI:

12:17 min

August 12, 2018

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Chapters

  • 00:00Title
  • 00:53Induction of Apoptosis
  • 02:34Apoptotic Body Isolation using FACS
  • 04:22ApoBD Isolation via Differential Centrifugation
  • 06:02Flow Cytometry Gating Strategy
  • 10:56Representative Result: Validation of Apoptosis
  • 11:16Representative Result: Comparison of ApoBD-Enriched Sample Purity
  • 11:47Conclusion

Summary

Automatic Translation

Een workflow met behulp van stroom cytometry of differentieel centrifugeren is ontwikkeld om te detecteren, te kwantificeren en te isoleren van apoptotic instanties uit een monster apoptotic hoge zuiverheid.

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