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검색 및 Apoptotic 몸 높은 순도 절연
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Detection and Isolation of Apoptotic Bodies to High Purity
DOI:

12:17 min

August 12, 2018

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Chapters

  • 00:00Title
  • 00:53Induction of Apoptosis
  • 02:34Apoptotic Body Isolation using FACS
  • 04:22ApoBD Isolation via Differential Centrifugation
  • 06:02Flow Cytometry Gating Strategy
  • 10:56Representative Result: Validation of Apoptosis
  • 11:16Representative Result: Comparison of ApoBD-Enriched Sample Purity
  • 11:47Conclusion

Summary

Automatic Translation

교류 cytometry 또는 차등 원심 분리를 사용 하 여 워크플로 검색, 계량 및 apoptotic 시체는 apoptotic 샘플 고 순도 분리 개발 된다.

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