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Journal
/
Developmental Biology
/
果蝇
S2R+ 细胞折叠-肠信号通路检测非肌球蛋白 II
. 收缩力的细胞基础研究
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
A Cell-based Assay to Investigate Non-muscle Myosin II Contractility
via
the Folded-gastrulation Signaling Pathway in
Drosophila
S2R+ Cells
Please note that all translations are automatically generated.
Click here for the English version.
果蝇
S2R+ 细胞折叠-肠信号通路检测非肌球蛋白 II
. 收缩力的细胞基础研究
DOI:
10.3791/58325-v
•
07:15 min
•
August 19, 2018
•
Kimberly A. Peters
,
Elizabeth Detmar
,
Liz Sepulveda
,
Corrina Del Valle
,
Ruth Valsquier
,
Anna Ritz
,
Stephen L. Rogers
3,4
,
Derek A. Applewhite
1
Department of Biology
,
The University of North Carolina at Chapel Hill
,
2
Department of Biology
,
Reed College
,
3
Carolina Center for Genome Sciences
,
The University of North Carolina at Chapel Hill
,
4
Lineberger Comprehensive Cancer Center
,
The University of North Carolina at Chapel Hill
Chapters
00:04
Title
01:10
Preparation of Concanavalin-A-coated Glass-bottomed Dishes and the Plating of S2R+ Cells
02:25
Efficacy Test of the Fog-conditioned Medium and the Cellular Contractility Assay
03:16
Fixing and Staining of Constricted S2R+ Cells
05:18
Imaging and Quantification of the Cellular Contractility Assay
05:41
Results: Post-treatment with Fog-Myc, RLC Forms Rings Indicative of Non-Muscle Myosin II Constriction
06:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里我们描述了一种使用
果蝇
S2R+ 细胞的收缩力测定方法。外源配体, 折叠肠 (雾) 的应用, 导致了雾信号通路和细胞收缩力的活化。该方法可用于研究雾信号通路中细胞收缩蛋白的调节。
Tags
Non-muscle Myosin II
Contractility
Folded-gastrulation Signaling Pathway
Drosophila S2R+ Cells
Cell-based Assay
Cell Density
Cell Morphology
Cell Shape Change
Morphogenesis
Development
Gene Discovery
RNAi Depletion
Article
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