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Journal
/
Biochemistry
/
寄生性锥虫
锥虫锥
虫的 f1-atpase 的分离
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Isolation of F
1
-ATPase from the Parasitic Protist
Trypanosoma brucei
Please note that all translations are automatically generated.
Click here for the English version.
寄生性锥虫
锥虫锥
虫的 f1-atpase 的分离
DOI:
10.3791/58334-v
•
08:44 min
•
January 22, 2019
•
Ondřej Gahura
,
Alena Zíková
2
1
Biology Centre
,
Czech Academy of Science, Institute of Parasitology
,
2
Faculty of Science
,
University of South Bohemia
Chapters
00:04
Title
00:57
Preparation of Sub-Mitochondrial Particles
02:43
Chloroform Release of F
1
-ATPase from the Membrane
04:08
Anion-Exchange Chromatography
05:34
Size-Exclusion Chromatography
06:49
Results: Two-Step Purification Results in Effective Isolation of F
1
-ATPase
08:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方案描述了从
锥虫
培养阶段的 f1-atpase 的纯化.
该程序产生了高度纯、均匀和活性的复合物, 适用于结构和酶学研究。
Tags
F1-ATPase
Trypanosoma Brucei
Mitoplasts
Protein Purification
Mitochondrial Membrane
Sonication
Ultracentrifugation
Chloroform Extraction
Dounce Homogenizer
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