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体小鼠膈肌三方突触细胞特异性钙信号的影像学研究
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm
DOI:

08:42 min

October 04, 2018

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Chapters

  • 00:04Title
  • 00:27Preparation of the Diaphragms and Phrenic Nerves from Transgenic Mice
  • 01:27Stimulation and Recording of the Muscle Action Potentials
  • 04:49Imaging of the Fluorescence of the Sample
  • 06:33Results: Nerve Stimulation-induced Ca2+ Responses in Populations of Schwann Cells or Muscle Cells of the Neonatal Mouse Diaphragm
  • 08:03Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个协议, 以图像钙信号在个别细胞类型的人群在小鼠神经肌肉连接。

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