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Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle
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Developmental Biology
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JoVE Journal Developmental Biology
Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle

Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle

DOI:
10.3791/58365-v

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08:33 min

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October 30, 2018

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, , , ,
1Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon, CNRS, 2Departments of Biochemistry and Molecular Biophysics, and Neuroscience, Mortimer B. Zuckerman Mind Brain Behavior Institute,Columbia University, 3Neuroscience Program,NYU School of Medicine

Chapters

  • 00:04Title
  • 00:29Leg Dissection and Fixation
  • 02:01Leg Mounting
  • 03:01Imaging and Post Imaging Processing
  • 07:08Results: Representative Drosophila Leg Motor Neuron Axon Imaging
  • 07:43Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a protocol to visualize the axonal targeting with a florescent protein in adult legs of Drosophila by fixation, mounting, imaging, and post-imaging steps.

Tags

DrosophilaLegMotor NeuronAxonsAdult CuticleFluorescent ProteinsAxon Visualization3D ReconstructionCarbon Dioxide AnesthesiaEthanolNon-ionic DetergentParaformaldehydeGlycerolMounting MediumMicroscope SlideCoverslip488 Nm Laser

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