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低血清条件下心脏祖细胞内皮分化的诱导作用
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Developmental Biology
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JoVE Journal Developmental Biology
Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions
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低血清条件下心脏祖细胞内皮分化的诱导作用

DOI:
10.3791/58370-v

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12:48 min

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January 07, 2019

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, , , ,
1Department of Biomedicine,University Hospital and University of Basel, 2Division of Cardiology,University Hospital Basel

Chapters

  • 00:04Title
  • 01:01Tissue Preparation and Digestion
  • 02:48Filtration
  • 04:43Primary Culture of Isolated SP-CPCs
  • 05:28SP-CPC Growth and Viability Under Different Serum Concentrations
  • 07:28Induction of Endothelial Differentiation
  • 08:59Tube Formation Assay
  • 10:15Results
  • 11:58Conclusion

Summary

Automatic Translation

Automatic Translation

该方案描述了一种用于心脏祖细胞的内皮分化技术。它特别关注血清浓度和细胞播种密度如何影响内皮分化潜力。

Tags

Cardiac Progenitor CellsEndothelial DifferentiationLow Serum ConditionsCell TherapyCardiac DiseaseAnesthetizeCollagenase BHank’s Balanced Salt SolutionRed Blood Cell Lysis Buffer

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