Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Developmental Biology
Author Produced

A subscription to JoVE is required to view this content.

Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions
 

Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

Article DOI: 10.3791/60843-v 10:24 min May 24th, 2020
May 24th, 2020

Chapters

Summary

In this protocol, we describe a stable, highly efficient differentiation strategy for the generation of postganglionic sympathetic neurons from human pluripotent stem cells. This model will make neurons available for the use of studies of multiple autonomic disorders.

Transcript

Tags

Differentiation Postganglionic Sympathetic Neurons Human Pluripotent Stem Cells Feeder-free Culture Conditions Chemically Defined Culture Conditions Sympathetic Nervous System Disorders Scalable Efficiency Electrically Active Neurons Disease Modeling Regenerative Medicine Drug Screening In Vitro Tissue Innovation Co-culture System Cardiac Tissue Regulation Confluency EDTA Treatment E8 Medium Vitronectin Coating
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter