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Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture
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JoVE Journal Developmental Biology
Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture

Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture

DOI:
10.3791/52823-v

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06:09 min

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May 14, 2015

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1Division of Cancer Research, College of Medicine, Dentistry and Nursing,University of Dundee

Chapters

  • 00:05Title
  • 01:10Plating the Cells
  • 02:56Immunofluorescent Staining
  • 04:13Results: Representative Neural Differentiation from Mouse Embryonic Stem Cell Cultures
  • 05:45Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes in detail the method for generating neural progenitors from embryonic stem cells using a serum-free monolayer method. These progenitors can be used to derive mature neural cell types or to study the process of neural specification and is amenable to multiwell format scaling for compound screening.

Tags

Neural DifferentiationMouse Embryonic Stem CellsSerum-free Monolayer CultureNeural ProgenitorsNeural SpecificationNeuronsGliaReporter LinesCell Culture

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