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JoVE Journal
Developmental Biology

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Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture
 

Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture

Article DOI: 10.3791/52823
May 14th, 2015

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Summary May 14th, 2015

This protocol describes in detail the method for generating neural progenitors from embryonic stem cells using a serum-free monolayer method. These progenitors can be used to derive mature neural cell types or to study the process of neural specification and is amenable to multiwell format scaling for compound screening.

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