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Developmental Biology
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低血清条件下で心臓前駆細胞の内皮への分化の誘導
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Developmental Biology
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JoVE Journal
Developmental Biology
Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions
Please note that all translations are automatically generated.
Click here for the English version.
低血清条件下で心臓前駆細胞の内皮への分化の誘導
DOI:
10.3791/58370-v
•
12:48 min
•
January 07, 2019
•
Michika Mochizuki*
1
,
Giacomo Della Verde*
1
,
Habiba Soliman
,
Otmar Pfister
2
,
Gabriela M. Kuster
2
1
Department of Biomedicine
,
University Hospital and University of Basel
,
2
Division of Cardiology
,
University Hospital Basel
Chapters
00:04
Title
01:01
Tissue Preparation and Digestion
02:48
Filtration
04:43
Primary Culture of Isolated SP-CPCs
05:28
SP-CPC Growth and Viability Under Different Serum Concentrations
07:28
Induction of Endothelial Differentiation
08:59
Tube Formation Assay
10:15
Results
11:58
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルでは、心臓前駆細胞の内皮への分化技術について説明します。特に、血清濃度と細胞播種密度が内皮細胞の分化に及ぼす影響について焦点を当てています。
Tags
Cardiac Progenitor Cells
Endothelial Differentiation
Low Serum Conditions
Cell Therapy
Cardiac Disease
Anesthetize
Collagenase B
Hank’s Balanced Salt Solution
Red Blood Cell Lysis Buffer
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