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低血清条件下で心臓前駆細胞の内皮への分化の誘導
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions
DOI:

12:48 min

January 07, 2019

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Chapters

  • 00:04Title
  • 01:01Tissue Preparation and Digestion
  • 02:48Filtration
  • 04:43Primary Culture of Isolated SP-CPCs
  • 05:28SP-CPC Growth and Viability Under Different Serum Concentrations
  • 07:28Induction of Endothelial Differentiation
  • 08:59Tube Formation Assay
  • 10:15Results
  • 11:58Conclusion

Summary

Automatic Translation

このプロトコルでは、心臓前駆細胞の内皮への分化技術について説明します。特に、血清濃度と細胞播種密度が内皮細胞の分化に及ぼす影響について焦点を当てています。

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