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Journal
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Immunology and Infection
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免疫沉淀法研究受体与酶的功能相互作用
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
Please note that all translations are automatically generated.
Click here for the English version.
免疫沉淀法研究受体与酶的功能相互作用
DOI:
10.3791/58433-v
•
09:40 min
•
September 28, 2018
•
Michael Peled
,
Marianne Strazza
,
Adam Mor
1
Pulmonary Department
,
The Chaim Sheba Medical Center
,
2
Columbia Center for Translational Immunology, Department of Medicine
,
Columbia University Medical Center
Chapters
00:04
Title
00:37
Transfection of Cells
01:54
Promoting Phosphorylation in Transfection Cells
02:40
Immunoprecipitation
06:13
Phosphatase Activity Assay
07:07
SHP2 Western Blot Analysis
08:16
Results: Analysis of Co-immunoprecipitation Assay
09:18
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们提出了一个共同免疫沉淀的协议和一个在珠酶活性检测, 同时研究的具体蛋白质领域的血浆膜受体对酶的招募和酶活性。
Tags
Co-immunoprecipitation
Receptor-enzyme Interaction
Cell Signaling
Protein Domains
Enzymatic Activation
DMEM
293-T Cells
Transfection
GFP Expression
Pervanadate
Phosphorylation
PD-1
Lysis Buffer
Protease Inhibitors
Sodium Orthovanadate
Immunoprecipitation
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